Author Affiliations
Abstract
1 Institute of Microscale Optoelectronics, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China
2 Shenzhen Key Laboratory of Ultraintense Laser and Advanced Material Technology, Center for Advanced Material Diagnostic Technology, College of Engineering Physics, Shenzhen Technology University, Shenzhen 518118, China
We demonstrate the generation of a unique regime of multiple solitons in a Tm-doped ultrafast fiber laser at ∼1938.72 nm. The temporal pulse-to-pulse separation among the multiple solitons, 10 in a single-pulse bunch, increases from 0.89 ns to 1.85 ns per round trip. In addition, with the increasing pump power, the number of bunched solitons increases from 3 up to 24 linearly, while the average time separation in the soliton bunch varies irregularly between ∼0.80 and ∼1.52 ns. These results contribute to a more profound comprehension of nonlinear pulse dynamics in ultrafast fiber lasers.
mode-locked fiber laser multiple solitons pump hysteresis pulse-to-pulse interval Chinese Optics Letters
2024, 22(3): 031405
1 江苏医药职业学院江宁临床医学院呼吸与危重症医学科，南京 211100
2 南京医科大学附属江宁医院药学部，南京 211100
3 南京市高淳人民医院呼吸与危重症医学科，南京 211300
硫利达嗪作为吩噻嗪类抗精神病类药物，具有诱导肿瘤发生免疫原性死亡（ ICD）、激活特异性免疫反应的潜力。本研究利用 A549和 H1299细胞探讨了硫利达嗪诱导肺腺癌细胞免疫原性死亡及其分子机制。将不同浓度的硫利达嗪与 A549和 H1299细胞共孵育 24 h后，利用四甲基偶氮唑蓝（ MTT）法检测细胞的存活及生长情况，利用流式细胞术分析细胞凋亡率，利用 ATP检测试剂盒检测细胞上清中的 ATP含量，利用免疫荧光法检测细胞表面钙网蛋白（ CRT）的表达，利用蛋白质印迹（ Western blot）法检测凋亡相关蛋白裂解的含半胱氨酸的天冬氨酸蛋白水解酶 3（Cleaved caspase 3）、B淋巴细胞瘤 -2（Bcl-2）、Bcl-2相关 X蛋白（ Bax）、细胞色素 C（Cyt C）的表达水平。结果表明，硫利达嗪能够显著抑制 A549和 H1299细胞的增殖，促进细胞凋亡，细胞增殖抑制及凋亡呈浓度依赖性。 ATP分泌量增加及细胞表面 CRT的表达水平上调，表明上述细胞发生了免疫原性死亡。而 Bcl-2表达水平下降， Bax和 Cyt C以及 Cleaved caspase 3表达水平上调，进一步证明了硫利达嗪可诱导肿瘤细胞凋亡。上述结果表明，硫利达嗪可通过线粒体应激信号通路诱导肺腺癌细胞发生免疫原性死亡，从而抑制肿瘤细胞的增殖。
肺腺癌 硫利达嗪 免疫原性死亡 线粒体应激信号通路 凋亡细胞 lung adenocarcinoma Thioridazine immunogenic cell death mitochondrial stress signaling pathway apoptotic cell
1 深圳大学微纳光电子学研究院二维材料光电科技国际合作联合实验室，广东 深圳 518060
2 深圳技术大学工程物理学院，广东 深圳 518118
3 深圳大学物理与光电工程学院光纤传感技术粤港联合研究中心，广东 深圳 518060
1.7 μm激光处于眼安全波段并位于许多重要气体分子的指纹吸收峰，在生物医疗、气体传感等领域具有重要应用价值。而涡旋光束作为一种新兴的结构光场，其具有环形光强分布和螺旋相位波前，并携带轨道角动量，在光通信、微粒操控等领域应用广泛。因此发展1.7 μm高能涡旋激光器具有重要的研究价值和应用前景。但传统稀土离子掺杂光纤或晶体的发射谱，或难以覆盖该波段，或在该波段激光增益较小，且涡旋光产生主要基于空间光结构，导致1.7 μm波段涡旋光激光系统复杂、集成度低，难以实现高功率输出。本文利用螺旋长周期光纤光栅作为涡旋模式转换器，在基于受激拉曼散射效应的1.7 μm波段光纤随机激光半开放腔中实现了全光纤结构的高功率涡旋激光输出，最大输出功率为2.09 W，中心波长为1690 nm。得益于涡旋光纤随机激光器的全光纤结构，该装置具有良好的时域稳定性，短时时域波动低至2.8%。该研究结果不仅为实现兼具高功率输出和良好时域稳定性的紧凑型1.7 μm波段涡旋激光器提供有效方案，还能进一步拓展其在激光医疗、气体检测、光镊和生物成像等领域的应用。
1.7 μm波段 涡旋光束 光纤随机激光器 螺旋长周期光纤光栅 涡旋光纤随机激光器 光学学报
2023, 43(22): 2214003
Author Affiliations
Abstract
Key Laboratory of Specialty Fiber Optics and Optical Access Networks, Joint International Research Laboratory of Specialty Fiber Optics and Advanced Communication, Shanghai Institute for Advanced Communication and Data Science, Shanghai University, Shanghai 200444, China
We have demonstrated the highly efficient excitation of the linearly polarized mode (LP01) in ring-core fibers (RCFs) by tapering the spliced point between the RCF and the standard single-mode fiber (SMF) to optimize all-fiber orbital angular momentum (OAM) generation. The tapering technique has been investigated theoretically and experimentally. Before tapering, only 50% of light can be coupled from SMFs to RCFs. The modal interference spectrum with an extinction ratio (ER) of ～9 dB is observed, showing that higher-order modes are excited in RCF. By tapering the spliced point, 90% of light is coupled, and the ER is minimized to be ～2 dB, indicating that the higher-order modes are effectively suppressed by tapering. Such tapered spliced points of RCF–SMF are further applied for all-fiber OAM generation. The efficiencies of OAM+1 and OAM?1 generation are found to be enhanced by approximately 11.66% and 12.41%, respectively, showing that the tapered spliced point of the RCF–SMF is a feasible way to optimize OAM generation.
ring-core fiber single-mode fiber all-fiber orbital angular momentum generation tapering technology Chinese Optics Letters
2020, 18(2): 020602
上海大学通信与信息工程学院特种光纤与光接入网重点实验室, 特种光纤与先进通信国际合作联合实验室,上海先进通信与数据科学研究院, 上海 200444
提出了一种在环形芯光纤中激发高阶涡旋光模式的方法,通过控制锥形微透镜单模光纤与环形芯光纤之间的倾斜角度和错位距离,实现了高阶涡旋光模式的激发。由数值模拟与实验测试结果可知,倾斜角度和错位距离分别约为8°和2 μm时,高质量的二阶涡旋光模式被激发。此外,由于锥形微透镜光纤具有聚焦光束的特性,其倾斜错位激发可以提高光的耦合效率,该方法与使用普通单模光纤的错位激发法相比,光的耦合效率提高13%左右。激发出的高阶涡旋光模式在高分辨率显微镜、光学微观操纵和光学传感等应用领域中具有巨大的应用潜力。
光纤光学 涡旋光 锥形微透镜单模光纤 环形芯光纤 轨道角动量
1 燕山大学电气工程学院, 河北 秦皇岛 066004
2 燕山大学信息科学与工程学院, 河北 秦皇岛 066004
将三维荧光光谱技术、小波压缩和交替惩罚三线性分解算法(APTLD)结合,提出了一种鉴别掺伪芝麻油的新方法。利用荧光光谱仪测量纯芝麻油及掺伪芝麻油样本的三维荧光光谱,通过激发校正和发射校正消除仪器带来的误差,得到样本的真实三维荧光光谱数据;利用小波压缩对处理后的真实数据进行压缩,以减少冗余信息,其中压缩分数和数据恢复分数分别大于94.00%和98.00%;利用APTLD算法对压缩后的数据进行定性及定量分析,得到的回收率为97.0%~99.8%,预测方均根误差不大于0.120。研究结果表明,所提方法能够准确鉴别纯芝麻油及掺伪芝麻油样本,并对其组分含量进行预测。
光谱学 三维荧光光谱 小波压缩 交替惩罚三线性分解 掺伪芝麻油鉴别
Author Affiliations
Abstract
1 New Drugs Innovation Institute Wuhan University of Science and Technology Wuhan 430065, P. R. China
2 Hubie Provincial Key Laboratory of Occupational Hazard Identification and Control Wuhan University of Science and Technology Wuhan 430065, P. R. China
3 Department of Pharmacy, Wuhan Asia Heart Hospital Wuhan, Hubei Province 430000, P. R. China
4 Department of Rehabilitation Medicine China Resources & WISCO General Hospital Wuhan 430080, P. R. China
5 Department of Biomedical Engineering School of Medicine and School of Engineering The University of Alabama at Birmingham Birmingham, AL 35294, USA
6 Shanghai University of Medicine and Health Sciences Shanghai 200000, P. R. China
To investigate the effect of Myocardin-related transcription factor A (MRTF-A) on apoptosis induced by ischemic/reperfusion (I/R), middle cerebral artery occlusion /reperfusion (MCAO/R) in rats were applied to mimic I/R. The neurological deficit score, cerebral infarct size, cortical neuron apoptosis and cleaved caspase 3 level were evaluated to determine the effect and the level of apoptosis by TTC straining, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) straining, Western blot and immunofluorescence staining. The myeloid cell leukemia-1 (Mcl-1) expression, release of cytochrome C (Cyt C) and its colocalization with apoptotic protease activating factor-1 (Apaf-1) were analyzed by quantitative real-time PCR (qRT-PCR), Western blot, and immunofluorescence staining. The results showed that MRTF-A overexpression could decrease the neurological deficit score and reduce cerebral infarct size (P < 0.01 versus Sham). In the MRTF-A-I/R group, TUNEL-positive cells and apoptosis ratio (%) (51.61 ± 6.17%) were significantly decreased compared to the Neg-I/R group (76.45 ± 8.77%) at 24 h reperfusion. Meanwhile, the cleaved caspase 3 expression revealed a similar trend while the expression of Mcl-1 was the opposite. Moreover, MRTF-A overexpression significantly enhanced Mcl-1 fluorescence intensity, which up-regulated the mRNA and protein level (P < 0.05 or P < 0.01 versus Neg-I/R). Furthermore, MRTF-A overexpression markedly inhibited the release of Cyt C, and decreased the colocalization with Apaf-1 in the cytoplasm (P < 0.05 or P < 0.01 versus Neg-I/R). All the data indicated that MRTF-A overexpression could improve the neurological function against cerebral I/R-induced apoptosis since underlying mechanism might be involved in the Mcl-1/Cyt C/cleaved caspase 3 signaling pathway.
Stroke animal model immunofluorescence staining laser scanning confocal microscope Journal of Innovative Optical Health Sciences
2019, 12(6):
1 燕山大学电气工程学院, 河北 秦皇岛 066004
2 燕山大学信息科学与工程学院, 河北 秦皇岛 066004
将三维荧光探测技术与交替惩罚三线性分解算法相结合,提出一种用于混合油液中油种成分检测的方法。首先利用FLS920型荧光光谱仪采集拟进行成分检测的混合油液(航空煤油和润滑油)在不同体积比配制条件下的20个样本的三维荧光光谱数据,利用Delaunay插值法对所获得的三维荧光光谱数据进行校正;然后利用核一致函数确定交替惩罚三线性分解算法解析三维荧光光谱数据时所需的组分数;最后利用均方根误差和相关系数矩阵,对交替惩罚三线性分解算法解析三维荧光光谱数据的效果进行评价。结果表明:在经交替惩罚三线性分解算法解析得到的均方根误差和相关系数矩阵中,非对角线上的元素值均满足所设阈值0.05和0.95的要求;在解决三维荧光光谱严重重叠的问题上,交替惩罚三线性分解算法优于平行因子算法,达到了对混合油液中油种成分快速检测的目的。
光谱学 油种检测 交替惩罚三线性分解 三维荧光光谱 光学学报
2018, 38(11): 1130005
山西农业大学动物科技学院, 山西 太谷 030801
提取35%VFI组、65%VFI组和自由采食组三个不同营养水平下的绵羊附睾头组织总RNA, 应用Real-Time PCR方法检测附睾头中GPx5 mRNA的表达规律, 利用免疫组化技术对附睾头GPx5进行定位分析。结果表明: GPx5 mRNA在附睾头的表达水平由低到高依次为: 35%VFI组<65%VFI组<自由采食组; 通过细胞免疫荧光分析检测到在附睾头上皮细胞有阳性产物。说明GPx5在不同营养水平下具有表达差异性, 低营养水平显著影响了附睾头GPx5的表达。
营养 附睾头 绵羊 nutrition GPx5 GPx5 caput epididymidis sheep
1 山西农业大学动物科技学院, 山西 太谷 030801
2 四川南江黄羊育种科学研究所, 四川 南江 636600
3 中国农业大学动物科技学院, 北京 100094
以405只南江黄羊为试验动物, 以生长激素(GH)为候选基因, 利用PCR-SSCP技术和DNA序列分析技术筛选其多态位点, 并与南江黄羊的生长性状进行关联分析, 以期筛选与南江黄羊生长性状相关的分子标记。序列分析比对结果发现GH基因外显子4有个4 SNPs (27 G/A、68 C/A、136 G/A、170 C/T), 其中有3个SNPs引起了氨基酸替代。与南江黄羊生长性状的关联分析结果显示: 外显子4多态与初生重、2月龄体重、6月龄体重和部分体尺指标显著相关(P<0.05); FF基因型是初生重、2月龄体重、6月龄体重、2月龄体长和2月龄胸围的有利基因型。GH基因外显子4的多态可能是与南江黄羊生长性状相关的分子标记, 但还需要在南江黄羊群体中进一步的验证。
生长激素基因 多态性 生长性能 南江黄羊 growth hormone gene polymorphisms growth traits Nanjiang Huang goats
